Rat ACAT1 (Acetyl Coenzyme A Acetyltransferase 1) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0574-48, ELK0574-96, ELK0574-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase

Assay Type: Sandwich

Sensitivity: 16.6 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ACAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ACAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ACAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ACAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P17764

Research Use Only